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Brilliantly Investigate DNA or RNA Stress Damage

Oxidative stress damage plays an important role in the etiology and progression of a multitude of human pathologies, including oncologic, cardiovascular, and autoimmune diseases and disorders. Through reactive oxygen species (ROS), oxidative stress can result in cellular damage, enzyme inactivation, and epigenetic impairment of DNA. A better understanding of oxidative stress damage—disease relationships can therefore be beneficial for diagnostic and therapeutic purposes. As a consequence, various biomarkers have been identified for oxidative stress damage, with the most well-studied being: oxidized nucleoside derivatives like 8-hydroxy-2’-deoxyguanosine (8-OH-dG) and 8-hydroxyguanosine (8-OH-G) that are generated by ROS in cellular DNA and RNA, respectively; the ROS hydrogen peroxide (H202) and its catalyzing enzyme superoxide dismutase (SOD); and histone H2AX phosphorylation, particularly at serine 139, which manifests during the DNA damage response.

Most available methods used to investigate these biomarkers are time-consuming and have low throughput, low accuracy, and high costs. By leveraging its EpiQuik™ technologies, EpiGentek has developed a series of innovative research tools to address those issues. Our comprehensive product suite for oxidative stress damage assessment includes the following features and advantages:

- Colorimetric/fluorometric ELISA-based assays with easy-to-follow steps for convenience and speed
- Fast procedure
- High sensitivity and specificity
- Assay flexibility via strip-well microplate format for either manual or high throughput analysis
- Simple, reliable, and consistent assay conditions

 
Exhibit Oxidative Stress Damage at the DNA Level Reveal Oxidative Stress Damage at the RNA Level Display Oxidative Stress Damage at the Cell/Tissue Extracts Level Show Oxidative Stress Damage at the In-situ Cell Level

DNA Damage Quantification Kits
Detect & Quantify Oxidative DNA Damage (8-OHdG) Directly from DNA

A few notable benefits include:

- Fast procedure can be completed within 3 hours and 45 minutes
- Detection limit can be as low as 1 pg of 8-OHdG
- High specificity by detecting only 8-OHdG without cross-reactivity to 8-OHdG analogues
- Highly correlated with and close to HPLC or LC-MS analysis
- Manual or high throughput capabilities
 
Catalog # Name Description
P-6003 EpiQuik 8-OHdG DNA Damage Quantification Direct Kit (Colorimetric) Colorimetric-based quantitation of 8-OH-dG in DNA samples (LOD = 2 pg of 8-OH-dG)
P-6004 EpiQuik 8-OHdG DNA Damage Quantification Direct Kit (Fluorometric) Fluorometric assay for quantitating 8-OH-dG in DNA samples (LOD = 1 pg of 8-OH-dG)

RNA Damage Quantification Kit
Directly Detect Oxidative RNA Damage (8-OhG) Status Using RNA

A few notable benefits include:

- Fast procedure can be completed within 3 hours
- Detection limit can be as low as 2 pg of 8-OhG
- High specificity by detecting only 8-OhG within the indicated concentration range of the sample RNA
- Uses RNA isolated from cells or tissues, no need for RNA digestion or hydrolysis
- Manual or high throughput capabilities
Catalog # Name Description
P-6008 EpiQuik 8-OhG RNA Damage Quantification Direct Kit (Colorimetric) Colorimetric-based quantitation of 8-OH-G in RNA samples (LOD = 2 pg of 8-OH-G)

Cell/Tissue Extract Oxidative Stress Damage Assays
Fast & Sensitive DNA Damage Measurement from Various Cells or Tissues

A few notable benefits include:

- Fast, easy-to-follow ELISA-like kits
- High sensitivity for detection from small input amounts
- Measurement from various samples
- Manual or high throughput capabilities
Catalog # Name Description
P-6005 EpiQuik In-situ and Ex-Situ Hydrogen Peroxide (H2O2) Assay Kit Fast and sensitive measurement of H2O2 from various samples, including living cells, cell/tissue extracts, and body fluids
OP-0001 EpiQuik Superoxide Dismutase Activity/Inhibition Assay Kit (Colorimetric) Detects SOD activity in cell/tissue extracts by measuring color production of a reaction mediated by the SOD substrate superoxide anion

 
In-Situ Oxidative Stress Damage Kits
Detect DNA Damage In-Situ from Various Samples

A few notable benefits include:

- Fast, easy-to-follow ELISA-like kits
- High sensitivity for detection from small input amounts
- Measurement from various samples
- Manual or high throughput capabilities
Catalog # Name Description
P-6001 EpiQuik In Situ DNA Damage Assay Kit Detects DNA damage or apoptosis by measuring phospho-H2AX (Ser139) in cultured adherent cells
P-6005 EpiQuik In-situ and Ex-Situ Hydrogen Peroxide (H2O2) Assay Kit Fast and sensitive measurement of H2O2 from various samples, including living cells, cell/tissue extracts, and body fluids
 

Info Epigentek

Epigenetic kits, antibodies and reagents for use in DNA methylation, histone modification, and chromatin studies.


Epigentek is the leading developer and provider of innovative technologies and products for epigenetic-related research. Founded in 2005, the company has since developed a comprehensive portfolio of proprietary products to provide complete and systematic solutions for epigenetics-related research and drug discovery.
Epigentek products are used all around the world by esteemed academia, leading pharmaceuticals, and cutting-edge biotechnology companies. These unique products are specifically designed to make assays much simpler, faster, more convenient, and highly more efficient than currently used methods.
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