AimPlex Biosciences

AimPlex Biosciences

News about AimPlex Biosciences

AimPlex BioSciences manufactures the AimPlex™ multiplex-assay kits for flow cytometry.

How It Works

AimPlex™ multiplex assay technology utilizes multiple bead populations differentiated by size and different levels of fluorescence intensity. With multiple sizes of beads and multiple levels of fluorescence intensity in each bead size, the AimPlex technology can measure up to 24 analytes simultaneously in a single reaction. The bead populations in the reaction are determined by a flow cytometer equipped with a 488nm laser. The maximum emission of the bead classification dye is at 700 nm.

Similar to the principle of a sandwich ELISA, each bead population conjugated with a specific capture antibody to trap the protein of interest, such as a cytokine, in the sample. The amount of the analyte captured is detected via a biotinylated antibody against a secondary epitope of the protein, followed by a streptavidin-R-phycoerythrin treatment. The fluorescent intensity of R-phycoerythrin on the beads is quantified on a flow cytometer. Concentrations of a protein of interest in the samples can be obtained by comparing the fluorescent signals to those of a standard curve generated from a serial dilution of a known concentration of the analyte.

The assay procedure consists of a 60 min antigen and capture antibody conjugated bead incubation step, a 30 min biotinylated detection incubation step and a 20 min streptavidin-PE incubation step.


Available AimPlex® Immunoassay Kit Formats

Single Plex Kits

All available AimPlex® immunoassays are grouped as 12-24 analytes per group. Each analyte in a group has a unique bead region and can be multiplexed together in any combination. Analytes in different groups may be multiplexed together if there is no conflicted on the bead regions but may have some cross-reactivity because we did not validate cross-reactivity of all the assays across different groups.

Each Single Plex Kit consists of 1 or more user selectable Analyte Kits, a Basic Kit and a sample matrix specific Diluent Kit. Each Analyte Kit contains analyte specific Ab conjugated beads, detection antibody(ies) and corresponding antigen standard(s). The Basic Kit has species-specific detection antibody diluent, reading buffer, wash buffer, Streptavidin-PE (SAPE), filter plate and plate seals. The Diluent Kit contains sample-type specific Standard Diluent and sample Assay Buffer.

Premixed Multiplex Kits
Each Premixed Multiplex Kit has a pre-defined multiplex panel with premixed antibody-conjugated beads, antigens and detection antibodies. Except the Reading Buffer (10x) and Wash Buffer (10x), all the reagents are supplied as ready-to-use.

Custom panels and assay development and custom bulk packaging
are also available. Please contact us for details.


All Available Assays click here.

Info AimPlex Biosciences

AimPlex® Multiplex Immunoassays for Flow™.


AimPlex Biosciences leverages their years of assay development expertise to offer comprehensive solutions in bioprocess development and biomedical testing. Founded in August 2011, the company is committed to developing and providing high-quality, cost-effective assay reagents for flow cytometry analyses.  Their products employ existing flow cytometry analytical platforms for detection. YSL Bio offers a rapidly-evolving, customizable test menu as well as services to develop new multiplex assay panels to improve your assay performance and efficiency.

AimPlex® multiplex assay technology utilizes multiple bead populations differentiated by size and different levels of fluorescence intensity. With multiple sizes of beads and multiple levels of fluorescence intensity in each bead size, the AimPlex® technology can measure up to 24 analytes simultaneously in a single reaction. The bead populations in the reaction are determined by a flow cytometer equipped with a 488nm laser. The maximum emission of the bead classification dye is at 700 nm.

For more information:
http://www.aimplexbio.com

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