AAT Bioquest

AAT Bioquest

News about AAT Bioquest

The CytoCite™ Fluorometer

The CytoCite™ Fluorometer is a palm-sized device designed for highly sensitive fluorescence-based quantitative analysis. This device, when paired with the appropriate bioassays, can be used to quantify biomolecules, proteins, nucleic acids, bacteria and other small molecules with a high degree of sensitivity, accuracy and repeatability. It can also be used as a standalone single-channel fluorometer for assay development.
 
CytoCite™' requires as little as 1 μL of sample volume, making it perfect for working with precious or limited samples.
As the only cloud-enabled fluorometer available, the CytoCite™ Fluorometer offers several key advantages over other similar products, such as Qubit™ and Nanodrop™ (both trademark of ThermoFisher), including unlimited storage of sample results and automated daily data backups to prevent unexpected data loss.  

Cytocite CBG100 User Manual
CBG100 CytoCite™ BG100 Portable Fluorometer For reading FITC channel
(Ex/Em= 480 nm/520 nm)
CGR100 CytoCite™ GR100 Portable Fluorometer For reading red fluorescence
(Ex/Em= 530 nm/620 nm)
 




Ratiometric Calcium Indicators: Performance & Selection Criteria
 
Ratiometric calcium indicators are designed to monitor calcium mobilization with greater accuracy and sensitivity. When bound to free Ca2+, the peak wavelength of either the excitation curve or emission curve of the ratiometric indicator will undergo a shift. This allows for very precise measurements of Ca2+ concentrations by correcting for uneven dye loading, dye leakage, and varying cell thicknesses.

For any experiment, the selection criteria for a ratiometric calcium indicator will depend on the instrument platform, the expected Ca2+ concentrations, and others. In general, it is useful to consider:

-  Calcium sensitivity and response
-  Filter set compatibility
-  Signal/background ratio
-  Cell retention (can vary by cell type)

Among ratiometric calcium indicators, Fura-2 and Indo-1 are the most widely used, although both have drawbacks in the form of only moderate signal/background ratio and poor cell retention. Fura-8™ demonstrates significant improvement in the signal/background ratio, and Fura-10™ outperforms both options and remains within cells significantly longer.
 


Image:

ATP-stimulated calcium response of endogenous P2Y receptor in CHO-K1 cells measured with Fura-2 AM, Fura-8™ AM and Fura-10™ AM in the absence of Probenecid:
CHO-K1 cells were seeded overnight, then Fura-2 AM or Fura-8™ AM or Fura-10™ AM without probenecid was added into the cells, and ATP was added to achieve the final indicated concentrations.

Check the product brochure for more information: Calcium Detection Probes & Assay Kits

Info AAT Bioquest

Advancing Assay & Test Technologies.


AAT Bioquest, Inc. develops, manufactures and markets bioanalytical research reagents and kits to scientists engaged in life sciences research, diagnostic R&D and drug discovery. They specialize in the area of photometric detections including absorption (color), fluorescence and luminescence technologies.

AAT Bioquest constantly introduces new products, and offers a rapidly expanding list of products that are grouped into several product lines.

1) Reactive fluorescent and luminescent probes, biotins and tag enzymes are used for labeling small drug molecules and biopolymers, e.g, proteins, nucleic acids and carbohydrates;
2) Fluorescent and luminescent probes for detecting proteins, nucleic acids and live cells;
3) Novel fluorescent and luminescent probes for detecting various enzymes, in particular, hydrolytic and redox enzymes;
4) Reagents for signal transduction research;
5) Physiological and neurological probes, e.g., calcium indicators and membrane potential probes.
Please use the contact us form for more information.