AAT Bioquest

AAT Bioquest

News about AAT Bioquest

Calbryte™ series

The Calbryte™ series is a family of fluorescent dyes developed to monitor intracellular calcium. It includes three novel calcium indictors:Calbryte™ 520Calbryte™ 590 and Calbryte™ 630. These indicators differ primarily in their excitation and emission wavelengths and can be detected using standard fluorescence instruments. The Calbryte™ series has been optimized for use with fluorescence microscopes, fluorescence microplate readers and flow cytometers. They can also be used in high-throughput screening applications. 
 

The Calbryte™ dyes have several key advantages over dyes like Fluo-3 and Fluo-4. Calbryte™ dyes generate much brighter signals, show significantly improved signal-to-background ratio and have greatly enhanced cellular retention. These qualities make the Calbryte™ series a superior choice over older, more traditional calcium indicators. 

What Calbryte™ dyes are currently available?

At present, Calbryte™ dyes are available in three different wavelengths: Calbryte™ 520, Calbryte™ 590 and Calbryte™ 630. 

Calbryte™ 520 is an upgrade for traditional green fluorescent indicators such as Fluo-3 and Fluo-4. This dye has an excitation maximum at 492 nm, which closely matches the standard 488 nm argon ion laser line. Also, with an emission maximum at 514 nm, this dye is compatible with the FITC filter sets found in most fluorescence instruments. Because Calbryte™ 520 has spectral values nearly identical to that of Fluo-4, no additional instrument setup is required. This allows for a seamless and convenient transition between products. 

Calbryte™ 590 is an upgrade for orange-red fluorescent indicators such as Calcium Orange™ and Rhod-2. This dye has an excitation maximum at 580 nm and is well excited by the 555 nm laser line. It has an emission maximum at 592 nm, making it compatible with TRITC/Cy3 filter sets. Calbryte™ 590 is approximately ten times more sensitive for calcium than Rhod-2, under comparable conditions. Moreover, unlike Rhod-2 which primarily localizes in mitochondria, Calbryte™ 590 retains well in the cytosol of cells. 

Calbryte™ 630 is an upgrade for red & deep-red fluorescent indicators such as X-Rhod-1. This dye has an excitation maximum at 608 nm, which aligns well with the 594 nm laser line. This dye has an emission maximum at 624 nm and is compatible with common Texas® Red filter sets. Because of its distance from the green region of the spectrum, Calbryte™ 630 is well suited for multiplex with a green fluorescent label such as iFluor 488Alexa Fluor 488 or GFP. Moreover, Calbryte™ 630's long emission wavelength makes it well suited for study of deep tissue. This is because longer wavelength dyes have an easier time penetrating through many cell layers, whereas short-wavelength dyes cannot. 

Info AAT Bioquest

Advancing Assay & Test Technologies.


AAT Bioquest, Inc. develops, manufactures and markets bioanalytical research reagents and kits to scientists engaged in life sciences research, diagnostic R&D and drug discovery. They specialize in the area of photometric detections including absorption (color), fluorescence and luminescence technologies.

AAT Bioquest constantly introduces new products, and offers a rapidly expanding list of products that are grouped into several product lines.

1) Reactive fluorescent and luminescent probes, biotins and tag enzymes are used for labeling small drug molecules and biopolymers, e.g, proteins, nucleic acids and carbohydrates;
2) Fluorescent and luminescent probes for detecting proteins, nucleic acids and live cells;
3) Novel fluorescent and luminescent probes for detecting various enzymes, in particular, hydrolytic and redox enzymes;
4) Reagents for signal transduction research;
5) Physiological and neurological probes, e.g., calcium indicators and membrane potential probes.

For more information:
http://www.aatbio.com

    (1930 product(s) found)

One moment please while the results are retrieved.

  • Product
    clone
    cat nr
    Download
  • 80001
    datasheet
    There is no technical datasheet available at the moment.
    Please enter your e-mail address below, so we can inform you when it's available.
    E-mail
      Subscribe to our newsletter
     
  • 15220
    datasheet
    There is no technical datasheet available at the moment.
    Please enter your e-mail address below, so we can inform you when it's available.
    E-mail
      Subscribe to our newsletter
     
  • 15221
    datasheet
    There is no technical datasheet available at the moment.
    Please enter your e-mail address below, so we can inform you when it's available.
    E-mail
      Subscribe to our newsletter
     
  • 4023
    datasheet
    There is no technical datasheet available at the moment.
    Please enter your e-mail address below, so we can inform you when it's available.
    E-mail
      Subscribe to our newsletter
     
  • 36702
    datasheet
    There is no technical datasheet available at the moment.
    Please enter your e-mail address below, so we can inform you when it's available.
    E-mail
      Subscribe to our newsletter
     
  • 40
    datasheet
    There is no technical datasheet available at the moment.
    Please enter your e-mail address below, so we can inform you when it's available.
    E-mail
      Subscribe to our newsletter
     
  • 4507
    datasheet
    There is no technical datasheet available at the moment.
    Please enter your e-mail address below, so we can inform you when it's available.
    E-mail
      Subscribe to our newsletter
     
  • 4022
    datasheet
    There is no technical datasheet available at the moment.
    Please enter your e-mail address below, so we can inform you when it's available.
    E-mail
      Subscribe to our newsletter
     
  • 14014
    datasheet
    There is no technical datasheet available at the moment.
    Please enter your e-mail address below, so we can inform you when it's available.
    E-mail
      Subscribe to our newsletter
     
  • 100
    datasheet
    There is no technical datasheet available at the moment.
    Please enter your e-mail address below, so we can inform you when it's available.
    E-mail
      Subscribe to our newsletter
     
    (1930 product(s) found)